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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark
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Packer, Witt and Tritschler, 1995 IV ALA achieves plasma concentrations that oral supplementation cannot approach, and its combination with glutathione and NAD+ in a comprehensive longevity protocol produces synergistic antioxidant and metabolic effects that each intervention achieves less effectively in isolation
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