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After 48 h of incubation under regular culture conditions the cells were used for fluorescence microscopy measurements
NAPstars specifically respond to dynamic NADP redox changes in vitro To further test the dynamic and specific responsiveness of NAPstars to changes in NADP redox status, we used recombinant NAPstar probes to monitor NADP dynamics in enzyme assays in vitro (Supplementary Fig
Vitamin C
Melanotan 2 retains the ability to bind to the melanocortin receptor but with increased affinity and longer duration due to resistance to enzymatic degradation, thus enabling supraphysiological levels of melanogenesis [5]
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