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FlpInCHO wildtype and mutant human GLP-1R cells were seeded at a density of 25 10 4 cells/well into 24-well culture plates and incubated overnight at 37 C in 5% CO 2 , washed three times in 1 PBS and fixed with 3.7% paraformaldehyde (PFA) at 4 C for 15 min

Storage Guidelines: Prior to reconstitution, the lyophilized powder should be stored away from light at -20C

Hers is similar to Noom (who I compared to Willow recently) in that they will advise you to take one of these other medications if they dont think GLP-1s are a fit

Furthermore, the oil red O staining showed it could be observed lipid droplets in some sections of HUA mice heart (Supplementary Figure 4F)