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C.TomoedaC
The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)
Our work expands our understanding of the downstream molecular events that are associated with antibiotic lethality, revealing cytoplasmic condensation as a phenotypic feature of antibiotic-induced bacterial cell death
But the site selection, skin preparation, angle, and rotation strategies are identical
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