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Ginsenoside Rg1 alleviates cholestatic liver injury by activating the SIRT1 pathway in hepatocytes
2017 Jul;55(6):569-572
For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)
The in-frame EGFP module at the C-terminal of the GST tag allows the positive cells to be observed under fluorescent microscopy and quantified with flow cytometry
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