Stock solutions of lipids were prepared at 10 mg/mL in chloroform for 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn- glycero-3-phosphor-1-serine (DOPS), or dissolved in chloroform at 0.2 mg/mL for 1,2-dioleoyl-sn-glycero-3-phospho-(1-myo-inositol-3-phosphate) (PtdIns(3)P) and 1,2-dioleoyl-sn-glycero-3-phospho-(1-myo-inositol-4-phosphate) (PtdIns(4)P)
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Follow with sunscreen during daytime this step protects your skin and helps the brightening process actually show results
Glutathione is a tri-peptide (L-g-glutamyl-L-cysteinyl-glycine) that acts as both reducing agent and capping agent, which assemble on the surface of AuNCs via a stable gold-thiol linkage (from cysteine) and imparts the nanoclusters with a net negative surface charge (zeta potential = 32 4.2 mV, pH = 7.5) due to the presence of terminal carboxylic acid functional groups (sketch depicting the assembly of glutathione molecules on GSH-AuNCs and the chemical structure of glutathione peptide is shown in Fig