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20-bp guide RNA sequences were designed using the CRISPR Design Tool ( and expressed along with Cas9 using the plentiCRISPRv2 vector, which was a gift from Feng Zhang (Addgene plasmid # 52961
It contains two types of vitamin C magnesium ascorbyl phosphate and ascorbyl glucoside, which I like because MAP is known for being more stable and gentle than l-ascorbic acid, as is acsorbyl glucoside
Key points for its efficacy include the following: Antioxidant Action: One of the syntheses from glutathione towards scavenging free radicals, consequently limiting the extent of oxidative damage, which comprises global cellular health
# 1706404, Bio-Rad) in Tris-buffered saline (TBS) (50 mM Tris, 150 mM NaCl, pH 7.5) containing 0.05% (wt/vol) Tween 20 (TBST) at room temperature
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