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For frozen sections, 4% paraformaldehyde-fixed brains were mildly shaken in 20% sucrose solution for 6 h and then 30% sucrose solution overnight at 4 C
The extract was then redissolved in 300 L of methanol-water (V:V = 1:4), vortexed for 30s, and sonicated for 3 min in an ice-water bath
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(has two N -i \) shows a second set of common reactions of lysine, including those used to attach a chromophore or a fluorescent label to the side chain
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