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In addition, the special regulatory characteristics of transcription factors also participate in the regulation of biofilm formation, for example, the phase separation ability of network transcription factors can precisely regulate the expression of downstream virulence-related genes, thereby directly affecting the formation of hyphae and biofilm generation processes (Ganser et al., 2023)

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Consider the example of the water-soluble bovine alpha-chymotrypsinogen, a 245 amino acid protein, whose sequence is shown below in single-letter code