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Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEPs mainly included nucleotide excision repair and p53 signaling pathway (Fig
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Denaturation and reduction steps were optimized to facilitate enzymatic digestion without compromising the lipidated moiety. Enzymatic Digestion A dual-enzyme approach employing Glu-C and chymotrypsin was utilized to generate overlapping peptide fragments, ensuring full sequence coverage
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