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Microscopy From rats, at end of the experiment, the brain, liver, kidney, stomach, duodenum, jejunum, colon, rectum, lungs, and heart were fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h
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Sperm with red-stained heads (PI-positive) were counted as having damaged membranes (nonviable), while all sperm with blue staining (Hoechst-positive and PI-negative) were counted as having undamaged membranes (viable) and a total of two hundred spermatozoa were evaluated for each sample
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