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The Office of Dietary Supplements states that none has been established because B12 is regarded as safe even at high doses, with the kidneys clearing the surplus into the urine
doi: 10.1038/s41573-023-00774-7 207 PackerM
Primary hippocampal neuron cultures are used to study Dihexa's proposed effects on dendritic spine density and synaptic protein expression, typically measuring morphological changes via fluorescence microscopy and quantifying synaptic marker expression (such as PSD-95 or synaptophysin) relative to untreated controls
This is due to several biological factors, including lower total body water, differences in alcohol dehydrogenase (ADH) activity, and hormonal influences 18
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