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HCs had no history of respiratory disease or recent respiratory tract infection and normal pulmonary function
Following incubation, cells were rinsed in PBS then incubated with WST-1 reagent (diluted 1:10 into fresh starved media (Roche, UK)) and incubated at 37 C for 4 h in a cell culture incubator according to manufacturers instructions
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Mix 1 to 2 mL of bacteriostatic water with the lyophilized BPC-157 powder
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