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As for my injection site of choice, I chose the upper buttocks (gluteus medius) mainly because of the size of the muscle and the relative ease I have accessing it
MDA-7 induces apoptosis via upregulation of the double-stranded-RNA dependent protein kinase (PKR)
For expression in yeast, these sequences were then transferred to pYES-DEST52 vector (Invitrogen, USA) through Gateway cloning technology using LR clonase-II enzyme following the manufacturers instructions
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