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Free radicals can damage renal tubular cells and disrupt the function of sodium transporters, leading to sodium retention and increased blood volume, which further elevates blood pressure [82]

21 DTNB Colorimetric Method Principle: DTNB reacts with the sulfhydryl group of glutathione to produce yellow-colored 5-thio-2-nitrobenzoic acid, which has a maximum absorption peak at a wavelength of 412 nm, while DTNB has almost no absorption peak above 400 nm