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The three biggest sources of waste are: Poor storage: Leaving reconstituted vials at room temperature accelerates degradation
For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
J Biol Chem 1997
All xenobiotics, including free radicals, pathogens and heavy metals, like mercury, stick onto glutathione (the SH in GSH is the sulfur-hydrogen ion attached to the molecule ) and are then carried out of the body through bile and stool
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