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TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturers instructions, and 210 l chloroform (Sigma Aldrich, Gillingham, UK) was added to each sample to obtain an aqueous phase
This is one reason pharmacists prefer concentrations that produce sub-1 mL doses
We typically prepare in-stock products submitted before the daily shipping cutoff (4 PM PST) for carrier collection the same business day
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