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Adesanya AW, Beauchamp MJ, Lavine MD, Lavine LC, Zhu F, Walsh DB

Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1