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Methylmalonic acid (MMA), a marker of vitamin B12 metabolism 33,34 , decreased significantly in the D + S group
Cell suspensions were filtered through 70-m cell strainers (Falcon, 352350), and two times the volume of erythrocyte lysis buffer (0.15 M NH 4 Cl, 10 mM KHCO 3 , 0.1 mM Na 2 EDTA, pH 7.27.4) was added for 5 min
In superficial areas such as the cheeks or jawline (fat thickness usually below 8 mm), long-term considerations change
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