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Cells were sub-cultured after treatment with 0.25% TrypsinEDTA once they reached coverage of 90%

Your body makes it naturally in the liver, and every single cell uses it.[1] Heres what makes it remarkable: glutathione exists in your cells at the same concentration as glucose, potassium, and cholesterol around 5 millimolar.[1] When something is that abundant, its not optional

GSH/GSSG ratio is an indication of cell redox status