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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)

The conjugate, which was linked via a disulfide bond exert an excellent effect and clearance rate for the conjugate, was about four times faster than that for the antibody component

However, the protocol recommendation to expose skin to UV within 46 hours post-injection conflicts with bedtime dosing, meaning users who inject before bed should plan UV exposure the following morning (812 hours post-injection) rather than immediately post-dose

Sanchez-Rodriguez and Mendoza-Nunez, 2019