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In the U.S., the Recommended Daily Allowance (RDA) for protein is about 0.36 grams per pound of body weight, or 54 grams per day for a 150-pound adult

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2.5 Liver triglyceride assay Frozen liver tissue samples were weighed (~100 mg) and homogenised by rapid agitation in 1 mL of phosphate-buffered saline with 1 mm diameter zirconia beads (BioSpec products, #11079110zx) using Precellys-Bertin homogeniser (Bertin Technologies)

Copper cycles between the divalent and monovalent states and, in the presence of a reductant and any peroxide, participates in Fenton-type chemistry that generates hydroxyl radicals