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Tolman, R
This upregulation exhausts intracellular GSH, leading to ROS buildup and oxidative DNA damage that can ultimately generate TP53 mutations, a process that is halted when CHAC1 is knocked down (Wada et al., 2018)
The principle of this method involves adding bait-specific antibodies and ProteinA-Turbo enzyme consecutively to permeabilized or nonpermeabilized cells
The expression of the antiapoptotic protein, Bcl-2 was also inhibited in GSH-depleted ASA treated cells
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