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We constructed a diabetic rat model using a previously reported method, administering a dose of 30 mg/kg intraperitoneally to induce type 1 diabetes ( 2.6 Immunohistochemistry Immunohistochemistry was used to detect the expression of GLP-1R in brain tissues of rats in different groups ( 2.7 qRT-PCR RT-PCR experiments were performed according to previously reported methods ( 2.8 ELISA kits The levels of antioxidant enzyme activities and inflammatory factors were measured by commercial kits ( 2.9 Blood glucose determination The method for measuring blood glucose was referred to the previous literature ( 2.10 Behavioral testing We followed the methods reported by previous researchers and used the elevated plus maze test, open field test and novel object recognition to evaluate the learning and cognitive abilities of rats ( 2.11 Date analysis All data were expressed as mean SD and statistically analyzed using GraphPad Prism V6.0

Furthermore, the ELF GSH levels that are maintained may in fact be tied to the CS induced depletion of GSH and that the lung may utilize GSH originating from other sources such as the liver and plasma to generate the high levels of ELF GSH

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Furthermore, GHK-Cu effectively reduced immune cell infiltration and alveolar permeability, critical hallmarks of ALI