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The presence of the specialized tripeptide KPV allows researchers to monitor the competitive downregulation of nuclear factor kappa B (NF-kB) and trace the inhibition of pro-inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) within inflamed intestinal or cutaneous tissues

Wu S, Chang G, Gao L, Jiang D, Wang L, Li G, et al

48 h post-transfection, cells were pelleted and resuspended (~1.5 g packed volume) into 5 mL membrane preparation buffer followed by homogenisation with a polytron homogeniser at 4 C