This advantage is thought to stem from tirzepatides unique ability to activate two hormones instead of just one, enhancing appetite suppression and metabolic benefits
Briefly, NIC cells seeded on Seahorse 96-well plates were cultured in substrate-limited DMEM ([-] D-glucose, [-] Sodium Pyruvate (Wisent, 319-062-CL)) with 0.5 mM D-glucose (BioShop #GLU501), 1 mM GlutaMAX (Sigma Aldrich, #G7021), 0.5 mM Carnitine (Sigma Aldrich, #C0283) and 1% FBS (Wisent, 080-150)
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Funcionan los pptidos GHK-Cu para las clulas madre