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Cells were incubated overnight in INS-1 832/3 media at 3 mM glucose 24 hours after cDNA and 48 hours after siRNA transfections and then pre-incubated for 30 minutes in KRBH buffer supplemented with 3 mM glucose before stimulation in KRBH buffer supplemented with 11 mM glucose 100 nM exendin-4 for 60 minutes

ADP-ribose interacts with the Mac1 hydrophobic cleft through conserved hydrogen bonds 68

Although the main action of GIP is to provide a stimulus for the release of insulin after an oral glucose challenge, the hormone has also been reported to promote lipid uptake in adipocytes [12]