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J Med Microbiol
Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates
Most users in the biohacking community report initial symptom improvement within 2-4 weeks
doi: 10.1007/978-1-4939-9597-4_1 17 MatejukA
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