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New collagen takes approximately four to six weeks to mature and integrate into existing tissue
Key points Semen analysis is a foundational but limited tool in assessing male fertility, which provides general insights into reproductive health through evaluation of sperm parameters (such as concentration, motility, morphology), but cannot definitively predict fertility
For the assessment of kidney injury severity (severity score), HE stained section images at 10 and 20 (2 at each magnification, for a total of 4 images per mouse) were graded from 0 (no damage) to 5 (most severe damage observed across the samples analyzed), based on the following parameters: (i) protein cast formation, (ii) visible tubular dilation, (iii) loss of proximal tubular cell polarization (no visible brush-border membranes) (iv) area affected (with single nephron damage to majority of the cortex with visual signs of injury)
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