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Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

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For instance, the YOLO type is motivated by things like impatience and influence from their peers, expresses little concern over possible side-effects of their AAS use, has low levels of knowledge about their drug use and is more likely to use a range of other drugs, including psychoactives