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Histology and Immunohistochemistry Paraformaldehyde-fixed and paraffin-embedded tissues were deparaffinized and rehydrated, then incubated with anti-Ter119 antibody (Biolegend, 116201, 1:200) at 4C overnight and with secondary Goat anti-Rat IgG (VECTOR, PI-9401) for 1 hour at room temperature
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Higher concentrations reduce injection volume but increase measurement errortargeting 5mg/mL allows precise dosing with standard insulin syringes calibrated in 0.01mL increments
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