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To isolate fractionated adipocytes, fresh gWAT was minced and digested for 1 h in PBS containing 10 mM CaCl 2 , 2.4 U ml 1 Dispase II (Roche) and 1.5 U ml 1 collagenase D (Roche)

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The first drug in this class based on Exendin-4 isolated from the gila monster, Byetta (shown above), was approved by the FDA in 2005

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