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GSTs are dimeric enzymes
So knowing what's in the bloodstream is only indicating how much B12 has not been absorbed or used by the body yet
The obtained areas under the total concentrationtime curve for drug in the brain and plasma (AUC total ) are used for calculation of K p,brain or can be combined with unbound fraction determined by equilibrium dialysis to calculate K p,uu,brain using a combinatory mapping approach (87, 97)
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