In this case, the glutathione anion with a charge of -2 transfers an electron to the methylcobalamin structure at the very beginning of the CASSCF(14,13) geometry optimization, breaking the Co-C bond, similarly to the interaction of methylcob(II)alamin with the dissociated homocysteine anion [2, 96, 97]
Step 4: Prepare the vial and syringe Remove the vials plastic cap and clean the top of the rubber stopper with an alcohol swab
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