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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]
Isoflavones have a structure like animal estrogens, such as estradiol-17, and exhibit affinity for estrogen receptors, classifying them as phytoestrogens with both estrogenic and anti-estrogenic effects (Sakthivelu et al., 2008)
Sawicka AK et al
revised the manuscript
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