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cDNA fragments encoding DIII of albumin with either of the naturally occurring amino acids, except for cysteine, in position 585, or QMP/LX, were synthesized and sub-cloned using the restriction enzyme sites BamHI and Xhol (GenScript)

On average, some individuals may start to notice weight loss within the first few weeks, though more significant results are typically seen after a few months of consistent use

The SDF files were converted to PDB format using Open Babel 2.4.1 27

We thank Christopher Koscher for his assistance with protein expression and purification and Florian Grebien for help with establishing the RPE-1 cell lines stably expressing p53 and p53 mutants